Exciting Research Opportunity at Harvard Medical School
An enthusiastic and highly-skilled research assistant is sought for an exciting opportunity in the Genetics Division of the Brigham and Women’s Hospital, Dana-Farber Cancer Institute, and Harvard Medical School. The candidate will be trained in cutting-edge methodologies including Nuc-Seq transcriptomics and CUT&RUN epigenomics to study novel regulators of pancreas development, inflammation, and cancer using mouse, zebrafish, and in vitro models. Knowledge gained from these studies will help pioneer novel interception and treatment strategies for pancreatic cancer.
Our research team (www.nissimlab.org) sits at a powerful and unique intersection of hereditary pancreatic cancer patient families in the clinic, new single cell technologies to study the pancreas, computational approaches for genome analysis, and animal and patient-derived models.
Candidates proficient in the following skills are preferred:
- Molecular biology techniques including cloning, DNA/RNA extraction, PCR, RT-PCR, western blot analysis
- Mouse husbandry, genotyping
- Tissue culture in vitro methods
Research experience with an outstanding academic record is required.
Under guidance, candidates will be highly encouraged to think critically about data, read scientific literature, and consider novel experimental directions. Candidates will also gain experience using zebrafish disease models.
This position will serve as a superb foundation for individuals interested in a career in medicine and/or biomedical research. Working in our group at Brigham and Women’s Hospital / Dana-Farber Cancer Institute / Harvard Medical School, together with close access to resources across the Boston biomedical community, offers unparalleled opportunity for research in an exciting, highly collaborative, and fun environment.
Principal Duties and Responsibilities:
1. With training and general supervision, performs daily activities of the laboratory, which include standard molecular biology techniques (cloning, DNA/RNA extraction, PCR, RT-PCR, western blot analysis), immunohistochemistry, confocal microscopy and imaging analysis, and CRISPR-Cas genome editing. These skills may be applied to new technologies including Nuc-Seq, 10x library preparation, and CUT&RUN.
2. Performs activities related to mouse care, including maintenance, genotyping, and breeding of relevant mouse lines.
3. Maintains tissue cultures and performs in vitro work.
4. Records data, summarizes, and analyzes results using basic statistics.
5. Prepares laboratory reagents and chemicals.
6. Maintains inventory and orders supplies when necessary.
7. Discusses progress of work regularly with supervisor.
8. All other duties, as assigned.
9. A commitment of 2 years is expected.
• Excellent organizational skills and meticulous attention to detail
• Enthusiasm and initiative to quickly learn new techniques and work independently
• Good manual dexterity and efficiency
• Ability to perform and prioritize tasks
• Professionalism; good interpersonal and communication skills
• Experience with molecular biology techniques, mouse husbandry, and tissue culture in vitro methods is preferred
(1) your CV
(2) a cover letter outlining your research interests and goals
(3) contact information for three references to Dr. Sahar Nissim, email@example.com.